Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin [HECD-1] antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human NSCLC Cell Line)
Specification
Human NSCLC Cell Line
Permeabilization
Yes - 0.3% Triton in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: 0.3% Triton, 5% Goat Serum in PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Life Technologies
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/500

Additional data

Additional Notes
Cells were grown in chamber slides. DNA counterstained with DAPI.
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Submitted May 06 2014

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