Immunocytochemistry/ Immunofluorescence abreview for Anti-E Cadherin antibody [HECD-1]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Human Cell (Renal tubular epithelial cells)
Specification
Renal tubular epithelial cells
Permeabilization
Yes - Triton X100, 0.5 %
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.01 % BSA/PBS
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/500

Additional data

Additional Notes
Primary epithelial cells were polarized on Millicell cell culture inserts. Cells of distal tublar origin stained positive for E-cadherin (green), nuclei are shown in red.
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Submitted Mar 19 2015

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