Western blot abreview for Anti-E Cadherin antibody [HECD-1]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (LNCaP - prostate cancer cells - plasma membrane fr)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
25 µg
Specification
LNCaP - prostate cancer cells - plasma membrane fr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% BSA in PBS
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-Ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: around 130 kDa
Username

Dr. Armen Petrosyan

Verified customer

Submitted Sep 26 2017

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