Western blot abreview for Anti-E Cadherin antibody [HECD-1]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-468 cells)
Loading amount
80 µg
Specification
MDA-MB-468 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
10 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
western lightning
Exposure
30 second(s)
Bands
Specific: 110 kDa

Additional data

Additional Notes
lane 1 and 2 whole cell extract of MDA-MB-468 (80µg)
Username

Dr. Alban Gaultier

Verified customer

Submitted Mar 23 2007

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