Overview

  • Product nameE-Cadherin (CDH1) Mouse ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 114.7 103% - 123%
    Serum 78.78 69% - 88%
    Plasma 88.93 76% - 95%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s E-Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E-Cadherin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse E-Cadherin coated on a 96- well plate. Standards and samples are pipetted into the wells and E-Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse E-Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E-Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceOne of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
  • Alternative names
    • Arc 1
    • Cadherin 1
    • cadherin 1 type 1 E-cadherin
    • Cadherin1
    • CAM 120/80
    • CD 234
    • CD324
    • CD324 antigen
    • CDH1
    • CDHE
    • ECAD
    • Epithelial cadherin
    • epithelial calcium dependant adhesion protein
    • LCAM
    • Liver cell adhesion molecule
    • UVO
    • Uvomorulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

E-Cadherin (CDH1) Mouse ELISA Kit images

  • Represntative Standard Curve using ab200678

  • Represntative Standard Curve using ab200678

Protocols

References for E-Cadherin (CDH1) Mouse ELISA Kit (ab100678)

ab100678 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"