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Tissue/ cell preparation (Escherichia coli ): Heat killed sonicate of E. coli (cocktail blend of strains of E. coli including the most commonly used DH5a and BL21).
Our Abpromise guarantee covers the use of ab25823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22427638|
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 24253282|
|WB||Use a concentration of 0.5 - 2 µg/ml. Note: Reacts with more than 50 proteins by Western Blot.|
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|