Overview

  • Product nameAnti-E. coli antibody (Biotin)
    See all E. coli primary antibodies
  • Description
    Rabbit polyclonal to E. coli (Biotin)
  • ConjugationBiotin
  • SpecificityMany "O" and "K" antigenic serotypes of Escherichia coli. Will remove E. coli proteins from recombinant preparations. Tested specifically with the strains O18, O20, O44, O55, O111, O112, O125, O157, K12.
  • Tested applicationsSuitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157).

  • General notes

    Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody

References for Anti-E. coli antibody (Biotin) (ab20640)

This product has been referenced in:
  • Kokkinis G  et al. A microfluidic, dual-purpose sensor for in vitro detection of Enterobacteriaceae and biotinylated antibodies. Lab Chip 16:1261-71 (2016). Read more (PubMed: 26939996) »
  • Yassine O  et al. Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip. Biomicrofluidics 8:034114 (2014). Read more (PubMed: 25379074) »

See all 12 Publications for this product

Product Wall

The biotinylation of ab20640 was accomplished by covalently coupling the primary antibody free amine groups with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Thank you very much for your reply.

I'm sending a free of charge vial of ab137967 on the order ***. This antibody is back-ordered until September 10, and I expect it to arrive shortly after that date.

Please note that as we have not...

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Thank you again for all of your patience while we've worked toadd the unconjugated rabbit antibody to our catalog.

This is antibody is now availableas Rabbit polyclonal to E. coli (ab137967)-

http://www.abcam.com/E-coli-antibody-ab1...

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Thank you for contacting Abcam earlier today.

I have talked to the lab about your enquiry regarding ab20640 and they have informed me that the antibody has been tested and is reactive with cell surface antigens on intact E. coli organisms. The...

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Thank you for contacting us. Unfortunately, the biotin/antibody ration is not determined for this antibody. As I mentioned, a common estimate for antibody biotinylation in general is is 3 - 6 biotin molecules per immunoglobulin. Please let us know if y...

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Thank you for contacting Abcam.
For ab25823, it should be fine to conjugate the antibody with biotin, using one of our EasyLink kits.
As for ab20640, it may react with BL21, but I cannot guarantee it, all we know is that it will recognize the...

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ab20640 is an IgG, so the MW without biotin is also ~150 kDa. I hope this helps.

Thank you for your enquiry. Unfortunately, it is not known exactly how much biotin per molecule of antibody is conjugated, the molecular weight of ab20640 is therefore undetermined. For ab35654, which is an unconjugated immunogloblulin, the molec...

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Thank you for your enquiry. I can confirm that ab20640 Anti-E. coli antibody (Biotin) will recognize the K12 strain of E. coli. This antibody has been tested in ELISA against E.coli serotypes O157:H7, O20, O55, O111, O125 and K12. It will there...

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Thank you for your enquiry. Unfortunately, it is not known exactly how much biotin per molecule of antibody is congjugated. I wish that I had more information and apologize for this inconveniance. I hope this information helps, please do not hesita...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"