Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.
Our Abpromise guarantee covers the use of ab30522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/25. Acetone fixation of the antigen is recommended.|