• Product nameAnti-E. coli antibody (FITC)
    See all E. coli primary antibodies
  • Description
    Rabbit polyclonal to E. coli (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (Escherichia coli, all serotypes). A mixed culture of E. coli strains (O157:H7, O20, O125, O55, O111 & K12), intact plus lysed, non-denatured, was gamma-inactivated and used directly as the immunogen.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 40% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab30522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/25. Acetone fixation of the antigen is recommended.


  • RelevanceEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
  • Alternative names
    • Escherichia coli antibody

References for Anti-E. coli antibody (FITC) (ab30522)

This product has been referenced in:
  • Golberg A  et al. Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli in water. PLoS One 9:e86341 (2014). Read more (PubMed: 24475107) »
  • Heinze BC & Yoon JY Nanoparticle immunoagglutination Rayleigh scatter assay to complement microparticle immunoagglutination Mie scatter assay in a microfluidic device. Colloids Surf B Biointerfaces 85:168-73 (2011). Read more (PubMed: 21411297) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Escherichia coli Cell (Planktonic)
Permeabilization Yes - acetone
Specification Planktonic
Fixation Acetone

Abcam user community

Verified customer

Submitted Aug 17 2015

Thank you for your enquiry.

It might be possible for a FITC-conjugated IgG to still be able to bind to Protein A as well, primarily due to the fact that FITC conjugation to IgG and Protein A binding to IgG occur at different sites within the...

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Thank you for your patience. I can now confirm that ab30522 also cross-reacts with the DH5a strain of E.coli. I hope this information is helpful for your customer.

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Escherichia coli Cell (pure culture)
Specification pure culture

Abcam user community

Verified customer

Submitted May 09 2007

Thank you for your patience. Regarding ab30522 (E.coli antibody (FITC)), a mixed culture of E. coli strains was gamma-inactivated and used directly as the immunogen. Although the concentration has not been determined, there is a dilution recommend...

Read More