Anti-E. coli heat labile toxin B antibody [BB2] (ab37203)

Overview

  • Product name
    Anti-E. coli heat labile toxin B antibody [BB2]
  • Description
    Mouse monoclonal [BB2] to E. coli heat labile toxin B
  • Specificity
    Esherichia coli heat labile enterotoxin B chain
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E.coli heat labile toxin, recombinant subunit B, expressed in E.coli.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purification notes
    Salt precipitation. Purity = 80 % (SDS-PAGE)
  • Clonality
    Monoclonal
  • Clone number
    BB2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.

Target

  • Relevance
    Escherichia coli heat-labile enterotoxin (LT) is the causative agent of traveller's diarrhoea, and it is also responsible for the deaths of hundreds of thousands of children per year in developing countries. LT is highly homologous in sequence, structure and function to cholera toxin (CT). Both toxins attack intestinal epithelial cells via specific binding to the branched pentasaccharide of ganglioside GM1 at the cell surface. LT is a plasmid-encoded, high molecular weight toxin, the crystalline structure of which revealed that it is composed of one A subunit (LTA) (27 kDa) and five non-covalently associated B subunits (LTB) (11.6 kDa each) forming a ring-like pentamer. The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase. The B chain has a membrane binding function. A receptor-binding antagonist which blocked the interaction of LT and CT with cell suface GM1 would potentially constitute a prophylactic drug conferring protection both against the severe effects of cholera itself and against the milder but more common disease caused by LT.
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • eltB antibody
      • Heat labile enterotoxin B chain antibody
      • LTB antibody
      • LTH B antibody
      • LTP B antibody
      see all

    References

    This product has been referenced in:
    • Becker PM  et al. Inhibition of binding of the AB5-type enterotoxins LT-I and cholera toxin to ganglioside GM1 by galactose-rich dietary components. Foodborne Pathog Dis 7:225-33 (2010). ELISA ; Escherichia coli . Read more (PubMed: 19919285) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    We have heaard back from the lab regarding epitope recognition of this product. Unfortunatley they have informed us that they have not mapped this epitope.

    I hope this information is helpful to you. ...

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    I am sorry for the delay in getting back to you.

    I now have further information to share with you from the lab in regards to the anti-E. coli heat labile toxin B antibody [BB12] (ab37204). The lab have retested this antibody as well as the ant...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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