Anti-E. coli heat labile toxin B antibody [BB2] (ab37203)

Overview

  • Product nameAnti-E. coli heat labile toxin B antibody [BB2]
  • Description
    Mouse monoclonal [BB2] to E. coli heat labile toxin B
  • SpecificityEsherichia coli heat labile enterotoxin B chain
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E.coli heat labile toxin, recombinant subunit B, expressed in E.coli.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purification notesSalt precipitation. Purity = 80 % (SDS-PAGE)
  • ClonalityMonoclonal
  • Clone numberBB2
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.

Target

  • RelevanceEscherichia coli heat-labile enterotoxin (LT) is the causative agent of traveller's diarrhoea, and it is also responsible for the deaths of hundreds of thousands of children per year in developing countries. LT is highly homologous in sequence, structure and function to cholera toxin (CT). Both toxins attack intestinal epithelial cells via specific binding to the branched pentasaccharide of ganglioside GM1 at the cell surface. LT is a plasmid-encoded, high molecular weight toxin, the crystalline structure of which revealed that it is composed of one A subunit (LTA) (27 kDa) and five non-covalently associated B subunits (LTB) (11.6 kDa each) forming a ring-like pentamer. The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase. The B chain has a membrane binding function. A receptor-binding antagonist which blocked the interaction of LT and CT with cell suface GM1 would potentially constitute a prophylactic drug conferring protection both against the severe effects of cholera itself and against the milder but more common disease caused by LT.
  • Cellular localizationSecreted
  • Database links
    • Alternative names
      • eltB antibody
      • Heat labile enterotoxin B chain antibody
      • LTB antibody
      • LTH B antibody
      • LTP B antibody
      see all

    References for Anti-E. coli heat labile toxin B antibody [BB2] (ab37203)

    This product has been referenced in:
    • Becker PM  et al. Inhibition of binding of the AB5-type enterotoxins LT-I and cholera toxin to ganglioside GM1 by galactose-rich dietary components. Foodborne Pathog Dis 7:225-33 (2010). ELISA ; Escherichia coli . Read more (PubMed: 19919285) »

    See 1 Publication for this product

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    Thank you for contacting us.

    We have heaard back from the lab regarding epitope recognition of this product. Unfortunatley they have informed us that they have not mapped this epitope.

    I hope this information is helpful to you. ...

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    I am sorry for the delay in getting back to you.

    I now have further information to share with you from the lab in regards to the anti-E. coli heat labile toxin B antibody [BB12] (ab37204). The lab have retested this antibody as well as the ant...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"