Mouse monoclonal [K99 12-444.5] to E. coli K99 pilus
SpecificityThese antibodies do not cross-react with K99-negative E. coli, E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDilute in PBS or medium which is identical to that used in the assay system.
These antibody may be used in an ELISA to detect and quantitate K99-positive E. coli.
ELISA titre is 1:16,000 (indirect assay against E. coli K99 pili on the solid phase. Titres represent endpoint dilutions that achieve absorbance values greater than 0.1).
RelevanceK99 pilus antigen is frequently associated with enterotoxigenic strains of E. coli isolated from the intestine of calves, sheep and pigs. Pilus antigens have been shown to be the main attachment mechanism whereby EPEC attach to and colonize the small intestine of neonatal animals. Any mechanism which will interfere with this attachment and colonization process is capable of reducing the intestinal colonization, diarrhoea and mortality associated with K99 EPEC infections.