Anti-E. coli K99 pilus antibody [K99 12-444.5] (ab1038)

Overview

  • Product nameAnti-E. coli K99 pilus antibody [K99 12-444.5]
    See all E. coli K99 pilus primary antibodies
  • Description
    Mouse monoclonal [K99 12-444.5] to E. coli K99 pilus
  • SpecificityThese antibodies do not cross-react with K99-negative E. coli, E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Purified E. coli K99 Pili.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS, pH7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberK99 12-444.5
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesDilute in PBS or medium which is identical to that used in the assay system.
    These antibody may be used in an ELISA to detect and quantitate K99-positive E. coli.
    ELISA titre is 1:16,000 (indirect assay against E. coli K99 pili on the solid phase. Titres represent endpoint dilutions that achieve absorbance values greater than 0.1).
  • Target

    • RelevanceK99 pilus antigen is frequently associated with enterotoxigenic strains of E. coli isolated from the intestine of calves, sheep and pigs. Pilus antigens have been shown to be the main attachment mechanism whereby EPEC attach to and colonize the small intestine of neonatal animals. Any mechanism which will interfere with this attachment and colonization process is capable of reducing the intestinal colonization, diarrhoea and mortality associated with K99 EPEC infections.
    • Cellular localizationCell Membrane
    • Database links
      • Alternative names
        • E.coli K99 protein antibody
        • Escherichia coli K99 pilus antibody
        • K99 pilus antibody

      References for Anti-E. coli K99 pilus antibody [K99 12-444.5] (ab1038)

      ab1038 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your enquiry. The clone number is K99 12-444.5. I have added this to the datasheet for future reference. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"