Overview

  • Product nameAnti-E. coli LPS antibody [2D7/1]
    See all E. coli LPS primary antibodies
  • Description
    Mouse monoclonal [2D7/1] to E. coli LPS
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation: Escherichia coli J5 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab35654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 18604629
ELISA 1/1500.
WB Use at an assay dependent concentration. PubMed: 22492977

Target

  • RelevanceSome form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock.
  • Alternative names
    • Escherichia coli LPS antibody

Anti-E. coli LPS antibody [2D7/1] images

  • ab35654 staining E. coli LPS in mouse hepatocytes (red). Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in SFX Kit blocking agent for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 555 goat anti-mouse, diluted 1/100. Cells were stimulated for 90 minutes with E. coli LPS and then probed with ab35654. The picture was taken on a Leica confocal microscope that pseudo-colors the 555 secondary red. The blue staining was with To-Pro-III Iodide,again, pseudo-colored to be blue on confocal.

    See Abreview

  • ab35654 staining E. coli LPS in rat intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate EDTA buffer pH 6.2. Samples were then blocked and incubated with ab35654 at a 1/100 dilution for 1 hour. The secondary used was a polyclonal goat anti-mouse IgG conjugated to alkaline phosphatase, used at a 1/100 dilution.

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References for Anti-E. coli LPS antibody [2D7/1] (ab35654)

This product has been referenced in:
  • Tokel O  et al. Portable microfluidic integrated plasmonic platform for pathogen detection. Sci Rep 5:9152 (2015). Read more (PubMed: 25801042) »
  • Nieto-Pelegrin E  et al. Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector. PLoS Pathog 10:e1004022 (2014). WB, ICC/IF ; Escherichia coli . Read more (PubMed: 24675776) »

See all 10 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Permeabilization Yes - TritonX-100
Specification Hela
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 19 2015

Application ELISA
Sample Mouse Serum (Intestinal fluid)
Specification Intestinal fluid
Type Sandwich (Capture)
Blocking step Pierce No Protein Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Sarah Hoyle

Verified customer

Submitted Nov 12 2013

Thank you for contacting us and your interest in our products.

The Anti-E. coli LPS antibody [2D7/1], ab35654, has been raised against whole E. coli cells (rather than disrupted or sonicated cells). Whilst specific epitope mapping has not been...

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Thank you for contacting us and your interest in the Anti-E. coli LPS antibody [2D7/1] (ab35654).

I now have further information to share in regards to how this antibody has been investigated for cross reactivity with LPS from other strains th...

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Thank you for your reply.

Unfortunately, we currently only have Pichia pastoris LPS protein (ab73792) available from our catalogue. However, we do not have an anti-Pichia pastoris LPS antibody available, I cannot therefore suggest a suitable p...

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Thank you for contacting us and your interest in our products.

The anti-E. coli LPS antibody [2D7/1], product code (ab35654), was raised against the J5 strain of E.coli, which produces incomplete LPS. The product you have purchased from xxxx i...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for m...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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The source of the antibody ab35654 informed us that unfortunately, we have not specifically tested this antibody against 0127:B8. Thus we currently do not have any empirical data to suggest it would react with this strain.

Thank you very much for your call today and your interest in these antibodies.

Since35654 has not been tested in EM, I can offer a discount off a future purchase if you buy ab35654 now, test it inEM, and submit feedback to us in the form of ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"