Overview

  • Product name
    Anti-E. coli LPS antibody [2D7/1]
    See all E. coli LPS primary antibodies
  • Description
    Mouse monoclonal [2D7/1] to E. coli LPS
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, IHC-P, IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation: Escherichia coli J5 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab35654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 18604629
ELISA 1/1500.
WB Use at an assay dependent concentration. PubMed: 22492977

Target

  • Relevance
    Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock.
  • Alternative names
    • Escherichia coli LPS antibody

Images

  • ab35654 staining E. coli LPS in mouse hepatocytes (red). Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in SFX Kit blocking agent for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 555 goat anti-mouse, diluted 1/100. Cells were stimulated for 90 minutes with E. coli LPS and then probed with ab35654. The picture was taken on a Leica confocal microscope that pseudo-colors the 555 secondary red. The blue staining was with To-Pro-III Iodide,again, pseudo-colored to be blue on confocal.

    See Abreview

  • ab35654 staining E. coli LPS in rat intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate EDTA buffer pH 6.2. Samples were then blocked and incubated with ab35654 at a 1/100 dilution for 1 hour. The secondary used was a polyclonal goat anti-mouse IgG conjugated to alkaline phosphatase, used at a 1/100 dilution.

    See Abreview

References

This product has been referenced in:
  • Motwani MP  et al. Prolonged immune alteration following resolution of acute inflammation in humans. PLoS One 12:e0186964 (2017). IHC-P ; Human . Read more (PubMed: 29073216) »
  • Svennerholm K  et al. Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction. Sci Rep 7:17434 (2017). IHC-P . Read more (PubMed: 29234030) »

See all 18 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (hemocyte with engulfed E. coli (DH5a))
Permeabilization
Yes - 0.1% Triton
Specification
hemocyte with engulfed E. coli (DH5a)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Catherine Brennan

Verified customer

Submitted Jan 10 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - TritonX-100
Specification
Hela
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 19 2015

Application
ELISA
Sample
Mouse Serum (Intestinal fluid)
Specification
Intestinal fluid
Type
Sandwich (Capture)
Blocking step
Pierce No Protein Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Sarah Hoyle

Verified customer

Submitted Nov 12 2013

Thank you for contacting us and your interest in our products.

The Anti-E. coli LPS antibody [2D7/1], ab35654, has been raised against whole E. coli cells (rather than disrupted or sonicated cells). Whilst specific epitope mapping has not been...

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Thank you for contacting us and your interest in the Anti-E. coli LPS antibody [2D7/1] (ab35654).

I now have further information to share in regards to how this antibody has been investigated for cross reactivity with LPS from other strains th...

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Thank you for your reply.

Unfortunately, we currently only have Pichia pastoris LPS protein (ab73792) available from our catalogue. However, we do not have an anti-Pichia pastoris LPS antibody available, I cannot therefore suggest a suitable p...

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Thank you for contacting us and your interest in our products.

The anti-E. coli LPS antibody [2D7/1], product code (ab35654), was raised against the J5 strain of E.coli, which produces incomplete LPS. The product you have purchased from xxxx i...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for m...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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The source of the antibody ab35654 informed us that unfortunately, we have not specifically tested this antibody against 0127:B8. Thus we currently do not have any empirical data to suggest it would react with this strain.

1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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