Anti-E. coli O + E. coli K antibody (ab31499)


  • Product nameAnti-E. coli O + E. coli K antibody
    See all E. coli O + E. coli K primary antibodies
  • Description
    Rabbit polyclonal to E. coli O + E. coli K
  • SpecificityReactive with all O and K antigenic serotypes of E. coli.
  • Tested applicationsSuitable for: Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Whole cell preparation E. coli, all serotypes



Our Abpromise guarantee covers the use of ab31499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA 1/2000.


  • RelevanceEscherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
  • Alternative names
    • Capsular antigen antibody
    • Escherichia coli antibody
    • Escherichia coli K antibody
    • Escherichia coli O antibody
    • K antigen antibody
    • O antigen antibody
    see all

References for Anti-E. coli O + E. coli K antibody (ab31499)

ab31499 has not yet been referenced specifically in any publications.

Product Wall

I'm glad you found the information of help.

If you do test any of these antibodies we would be very interested to hear of the results, regardless of if they are positive or not. We like to encourage all of our customers to submit an Abreview ...

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The immunogen was a mixture of intact and lysed bacteria which had not been denatured in any way. This antibody is reactive with all O and K antigenic serotypes of E. coli, therefore it will recognize the K12 strain. This antibody is purified by a...

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Thank you for contacting us. I have been in touch with the lab regarding your inquiry. I do wish to apologize for the delay in getting this information for you. Concerning ab31499, Anti-E. coli O + E. coli K antibody: The immunogen for th...

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Thank you for contacting us and your interest in our products.

I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Escherichia coli Cell (Bacterial over night culture of E.coli HB101)
Specification Bacterial over night culture of E.coli HB101
Preparation Cell harvesting/tissue preparation method: washed with PPS
Sample buffer: PBS containing 10% FCS, 1% BSA
Fixation PFA 0,4% after staining
Permeabilization No
Gating Strategy total events (FSC, SSC log)

Herr Dr. Jonathan Jantsch

Verified customer

Submitted Feb 03 2010