FITC Anti-E. coli O + E. coli K antibody (ab20856)
Key features and details
- FITC Goat polyclonal to E. coli O + E. coli K
- Suitable for: ICC/IF
- Reacts with: Escherichia coli
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-E. coli O + E. coli K antibody
See all E. coli O + E. coli K primary antibodies -
Description
FITC Goat polyclonal to E. coli O + E. coli K -
Host species
Goat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Tissue, cells or virus corresponding to Escherichia coli E. coli O + E. coli K. Mixture of E. coli serotypes (E. coli).
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General notes
The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Notes |
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Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Escherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens. -
Alternative names
- Capsular antigen antibody
- Escherichia coli antibody
- Escherichia coli K antibody
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Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab20856 has been referenced in 1 publication.
- Zhao WD et al. Caspr1 is a host receptor for meningitis-causing Escherichia coli. Nat Commun 9:2296 (2018). PubMed: 29895952