Anti-E. coli O + E. coli K antibody (FITC) (ab20856)


  • Product nameAnti-E. coli O + E. coli K antibody (FITC)
    See all E. coli O + E. coli K primary antibodies
  • Description
    Goat polyclonal to E. coli O + E. coli K (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityMany "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is not absorbed and may react with related Enterobacteriaceae.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Mixture of E. coli serotypes (E. coli).

  • General notes

    The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.



Our Abpromise guarantee covers the use of ab20856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIF: Use at an assay dependent dilution.
    Acetone fixation of the antigen source is recommended prior to staining. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceEscherichia coli (E. coli) are Gram negative enteric bacteria that live in the human gut. Its presence in nature is an indication of human feacal pollution. E. coli also causes human disease such as urinary tract infections and neonatal meningitis. Infection with particular enterotoxigenic strains can lead to life threatening intestinal diseases. E. coli are distinguished immunologically by serotyping based on three types of antigen: the somatic or O antigen which corresponds to terminal sugars on the cell surface lipopolysaccharide, the capsular or K antigens and the flagellar or H antigens.
    • Alternative names
      • Capsular antigen antibody
      • Escherichia coli antibody
      • Escherichia coli K antibody
      • Escherichia coli O antibody
      • K antigen antibody
      • O antigen antibody
      see all

    References for Anti-E. coli O + E. coli K antibody (FITC) (ab20856)

    ab20856 has not yet been referenced specifically in any publications.

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    I'm glad you found the information of help.

    If you do test any of these antibodies we would be very interested to hear of the results, regardless of if they are positive or not. We like to encourage all of our customers to submit an Abreview ...

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    Thank you for contacting us and your interest in our products.

    I can now share some more information relating to the antibodies we have directed towards E. coli (O and K). We have two different antibodies directed against this bacterium, with...

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    Thank you for your enquiry. Good news: In ab20856, the FITC is conjugated to the amine group. Please let me know if you need anything further.

    Thank you for your enquiry. Ab20856 is reactive with E. coli O157 but has not been tested with K99. I hope this information helps. Please do not hesitate to contact us again if you need anything further.