Anti-E. coli O152 antibody (ab78978)


  • Product nameAnti-E. coli O152 antibody
  • Description
    Rabbit polyclonal to E. coli O152
  • SpecificitySpecific to E. coli somatic O152 antigen
  • Tested applicationsSuitable for: Agglutination, ICC/IFmore details
  • Species reactivity
    Reacts with E. coli O152.
  • Immunogen

    Tissue/ cell preparation of E. coli O152 (heat inactivated).

  • Positive control
    • Antigenic suspension.
  • General notesReagents should be allowed to stand at 15°C - 25°C for at least 30 minutes prior to use.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab78978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.

See attached protocol. Use heat inactivated bacteria.

ICC/IF Use at an assay dependent concentration.


  • RelevanceEscherichia coli are a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli. These are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E.coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requries verification of their pathogenicity, pathogenic E. coli have often specific serotypes; therefore, typing of the serogroup and serotype is necessary to screening pathogenic E. coli.

Anti-E. coli O152 antibody images

  • ab78978 staining E.coli in mouse colon tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were snap frozen without fixation and incubated in 1% PFA in PBS after cutting sections (5µm). Sections were permeabilized with 0.5% NP40 and blocked with 10% goat serum for 1 hour at 25°C. Sections were incubated with primary antibody (1/500 in 5% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-E. coli O152 antibody (ab78978)

ab78978 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Cell (mouse colon sections (snap frozen))
Specification mouse colon sections (snap frozen)
Permeabilization Yes - 0.5% NP40
Fixative snap frozen without fixation 1% PFA in PBS after cutting sections

Dr. Jerry Turner

Verified customer

Submitted Feb 27 2014