Anti-E. coli O152 antibody (ab78978)

Overview

  • Product name
    Anti-E. coli O152 antibody
  • Description
    Rabbit polyclonal to E. coli O152
  • Specificity
    Specific to E. coli somatic O152 antigen
  • Tested applications
    Suitable for: Agglutination, ICC/IFmore details
  • Species reactivity
    Reacts with E. coli O152.
  • Immunogen

    Tissue/ cell preparation of E. coli O152 (heat inactivated).

  • Positive control
    • Antigenic suspension.
  • General notes
    Reagents should be allowed to stand at 15°C - 25°C for at least 30 minutes prior to use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration.

See attached protocol. Use heat inactivated bacteria.

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Escherichia coli are a group of gram-negative bacilli belonging to the family Enterobacteriaceae and is considered as one of the bacteria in the human intesinal normal microbial flora. Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigen (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli. These are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E.coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requries verification of their pathogenicity, pathogenic E. coli have often specific serotypes; therefore, typing of the serogroup and serotype is necessary to screening pathogenic E. coli.

Images

  • ab78978 staining E.coli in mouse colon tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were snap frozen without fixation and incubated in 1% PFA in PBS after cutting sections (5µm). Sections were permeabilized with 0.5% NP40 and blocked with 10% goat serum for 1 hour at 25°C. Sections were incubated with primary antibody (1/500 in 5% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

ab78978 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (mouse colon sections (snap frozen))
Specification
mouse colon sections (snap frozen)
Permeabilization
Yes - 0.5% NP40
Fixative
snap frozen without fixation 1% PFA in PBS after cutting sections
Username

Dr. Jerry Turner

Verified customer

Submitted Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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