Anti-E. coli O157 antibody [1031] (ab13625)

Overview

  • Product nameAnti-E. coli O157 antibody [1031]
    See all E. coli O157 primary antibodies
  • Description
    Mouse monoclonal [1031] to E. coli O157
  • SpecificityAb13625 is specific for E. coli serotype O157 and immunoprecipitates LPS of O157:H7.
  • Tested applicationsSuitable for: ELISA, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Tissue/ cell preparation (E. coli).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
  • ClonalityMonoclonal
  • Clone number1031
  • IsotypeIgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
IP
  • Application notesELISA: 1/20 - 1/200. Functions as a capture antibody, detector antibody IF: 1/10 - 1/50.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceEscherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
    • Alternative names
      • Escherichia coli O157 antibody

    References for Anti-E. coli O157 antibody [1031] (ab13625)

    ab13625 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"