Overview

  • Product name
    Anti-E. coli O157 antibody [3011]
    See all E. coli O157 primary antibodies
  • Description
    Mouse monoclonal [3011] to E. coli O157
  • Specificity
    Specific for E. coli serotype O157. The region recognized by this antibody is LPS oligosaccharide. Non-reactive with serotypes: O111, O125, O20 and O55 and K12.
  • Tested applications
    Suitable for: IP, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E. coli 0157 whole cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure.
  • Clonality
    Monoclonal
  • Clone number
    3011
  • Myeloma
    unknown
  • Isotype
    IgG3
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. Immunoprecipitates LPS of O157:H7. May be conjugated to colloidal gold.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. May be used as either a capture or detection antibody.
IHC-P Use at an assay dependent concentration. PubMed: 21858177

Target

  • Relevance
    Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • Alternative names
    • Escherichia coli O157 antibody

References for Anti-E. coli O157 antibody [3011] (ab20976)

This product has been referenced in:
  • Li Y  et al. An Electrochemical Strategy using Multifunctional Nanoconjugates for Efficient Simultaneous Detection of Escherichia coli O157: H7 and Vibrio cholerae O1. Theranostics 7:935-944 (2017). Read more (PubMed: 28382165) »
  • Etienne-Mesmin L  et al. Interactions with M Cells and Macrophages as Key Steps in the Pathogenesis of Enterohemorragic Escherichia coli Infections. PLoS One 6:e23594 (2011). IHC-P . Read more (PubMed: 21858177) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

We would not be able to confirm the DH5alpha specifictiy of ab37204 and ab20976. However we can confirm ab25823 and ab48416 would work with DH5alpha species.

I hope this information is helpful to you. Plea...

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I'm happy to help. Please let me know if you have any further questions.

Thank you for your enquiry. We typically do not have IEF data for our antibodies. I have found this reference for you that might be of some help: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150060305/abstract Please let me know if you need anyth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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