• Product nameAnti-E. coli O157 antibody [3011]
    See all E. coli O157 primary antibodies
  • Description
    Mouse monoclonal [3011] to E. coli O157
  • SpecificitySpecific for E. coli serotype O157. The region recognized by this antibody is LPS oligosaccharide. Non-reactive with serotypes: O111, O125, O20 and O55 and K12.
  • Tested applicationsSuitable for: IP, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    E. coli 0157 whole cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure.
  • ClonalityMonoclonal
  • Clone number3011
  • Myelomaunknown
  • IsotypeIgG3
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab20976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. Immunoprecipitates LPS of O157:H7. May be conjugated to colloidal gold.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. May be used as either a capture or detection antibody.
IHC-P Use at an assay dependent concentration. PubMed: 21858177


  • RelevanceEscherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • Alternative names
    • Escherichia coli O157 antibody

References for Anti-E. coli O157 antibody [3011] (ab20976)

This product has been referenced in:
  • Etienne-Mesmin L  et al. Interactions with M Cells and Macrophages as Key Steps in the Pathogenesis of Enterohemorragic Escherichia coli Infections. PLoS One 6:e23594 (2011). IHC-P . Read more (PubMed: 21858177) »
  • Park S  et al. Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157:H7. Ultramicroscopy 108:1348-51 (2008). Read more (PubMed: 18562109) »

See all 3 Publications for this product

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We would not be able to confirm the DH5alpha specifictiy of ab37204 and ab20976. However we can confirm ab25823 and ab48416 would work with DH5alpha species.

I hope this information is helpful to you. Plea...

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I'm happy to help. Please let me know if you have any further questions.

Thank you for your enquiry. We typically do not have IEF data for our antibodies. I have found this reference for you that might be of some help: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150060305/abstract Please let me know if you need anyth...

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