Overview

  • Product name
    Anti-E. coli O157 antibody (FITC)
    See all E. coli O157 primary antibodies
  • Description
    Goat polyclonal to E. coli O157 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Highly specific for E. coli O157:H7
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Whole cells of E. coli serotype O157:H7

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab31550 is an affinity purified IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20 - 1/2000.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
  • Alternative names
    • Escherichia coli O157 antibody

References

This product has been referenced in:
  • Battle SE  et al. Actin pedestal formation by enterohemorrhagic Escherichia coli enhances bacterial host cell attachment and concomitant type III translocation. Infect Immun 82:3713-22 (2014). ICC/IF . Read more (PubMed: 24958711) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your enquiry regarding ab31550. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

I would like to reassure you that our Abpromise applies to your complaint since you...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up