The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae, bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. Toxigenic E. coli possess special pili which are antigenetically different from those of other E. coli.
Pili are macromolecular structures that are responsible for the attachment and infection of the organism to the intestinal mucous epithelial cells and also the ability to produce enterotoxin.