E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

Q&A (5)Specific References (2)
Customer reviews and FAQs

Overview

  • Product nameE. coli Positive Control (Escherichia coli ) Whole Cell Lysate
  • General notes

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
    T7 HSV
    C-Myc
    VSV-g
    Glu-Glu
    V5
    E-tag
    FLAG (DDDDK)
    S-tag
    HA
    6x His

    GST

    Tag sequences: T7 = CMASMTGGQQMG
    HSV = QPELAPEDPEDC
    CMYC = EQKLISEEDLC
    VSV-g = YTDIEMNRLGK
    GluGlu = EYMPME
    V5 = GKPIPNPLLGLDST
    E-tag = GAPVPYPDPLEPR
    FLAG = DYKDDDDK
    HA = YPYDVPDYAC
    6xHIS = HHHHHH

     

    Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundEscherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

Applications

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

E. coli Positive Control (Escherichia coli ) Whole Cell Lysate images

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl
  • Western blot
    Western blot
    Anti-Myc tag antibody (ab107247) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

References for E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

This product has been referenced in:
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). Read more (PubMed: 24278455) »
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . Read more (PubMed: 21515633) »

See all 2 Publications for this product

Publishing research using ab5395? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit a question for ab5395

5 Q&A

10
Votes: Highest First

Hey,

Is the concentration of the tagged fusion protein known? If not, do you sell any myc-tagged fusion proteins where the concentration is known?

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesit...

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J’aurais besoin de savoir quelle est la souche d’E.Coli utilisée pour le lysat ab5395 (concernant le lysat ab2431 la souche utilisé est BL21).

Merci beaucoup pour votre retour

Cordialement

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très d...

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Do you have the sequence of each of the tags contained in the recombinant protein?

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC...

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Customer reported receiving 27 ul instead of 50 ul in their vial of ab5395. Order# 88221.

Thank you for your phone call and I apologize again for the shortage that you received in your vial of ab5395. I have arranged for a free of charge replacement to be sent to you; it is on order# 88591 and you should receive this Tuesday. Please let ...

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We have a claim about #ab5395(E.coli positive control Lysate containing 11 different epitope tags). We received this products this morning. But then the vial we saw was black. This black block seemed the fungus. Is this product color black? We send p...

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Thank you for your enquiry. E. coli were lysed with SDS-PAGE sample buffer containing loading dye (ready for Western blot analysis) which gives the dark colour. Please take a look at the datasheet especially the Purification notes. Should your c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"