This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
T7 HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
GST
Tag sequences: T7 = CMASMTGGQQMG
HSV = QPELAPEDPEDC
CMYC = EQKLISEEDLC
VSV-g = YTDIEMNRLGK
GluGlu = EYMPME
V5 = GKPIPNPLLGLDST
E-tag = GAPVPYPDPLEPR
FLAG = DYKDDDDK
HA = YPYDVPDYAC
6xHIS = HHHHHH
Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.
Our Abpromise guarantee covers the use of ab5395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use at an assay dependent concentration. Use 0.5 - 2.0 µl for 10 - 12% gels. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"