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This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.
The cell lysate was prepared from E.coli (Tuner [De3]) expressing a 14.2 kDa protein containing a C-terminal 6xHis tag.
Our Abpromise guarantee covers the use of ab2431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. This E. coli (strain BL21)lysate contains a recombinant C-terminal 6x-His tagged protein of 17 kDa. The his-tagged protein is monomeric and will be detected under reducing and non-reducing conditions. Use 5-20 µg per lane of a mini-gel at 12-15 % SDS-PAGE. After the lyophilised pellet is dissolved in 250 ul SDS-gel sample buffer (1 mg/ml protein lysate) it can be used direct for sample loading and than stored at -20°C. The sample can be reused several times.|
Western blot of 9