Overview

  • Product nameE-selectin Monkey ELISA KitSee all E-selectin kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4 < 5%
    Overall 4 < 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 < 10%
    Overall 4 < 10%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.4 U/ml
  • Range
    1.9 U/ml - 60 U/ml
  • Recovery

    85 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.

    E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P16581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CD62 antigen-like family member E
    • CD62E
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial adhesion molecule 1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    • Selectin E
    see all

Applications

Our Abpromise guarantee covers the use of ab119523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

E-selectin Monkey ELISA Kit images

  • Representative Standard Curve using ab119523.

Protocols

References for E-selectin Monkey ELISA Kit (ab119523)

ab119523 has not yet been referenced specifically in any publications.

Product Wall

The product has 120 days guarantee period in Thailand. The 120 days starts form the date of purchase. Expiry date for biological compounds is difficult to guess so we provide guarantee rather than expiry date.
Please store the components as advise...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"