Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. For colorimetric techniques use at a dilution of 1/1000 - 1/10000 and for chemiluminescent techniques use at a dilution of 1/1000 - 1/30000.
ICC 1/100 - 1/400.
ELISA Use at an assay dependent concentration. For coating techniques use at a dilution of 1/100 - 1/500 abd for primary techniques use at a dilution of 1/1000 - 1/30000

Target

  • Relevance
    E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
  • Alternative names
    • E epitope tag antibody
    • GAPVPYPDPLEPR epitope tag antibody
    • GAPVPYPDPLEPR tag antibody

References

This product has been referenced in:
  • Mont S  et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . Read more (PubMed: 27118599) »
  • Ouchi T  et al. Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome. J Exp Med 208:2607-13 (2011). WB . Read more (PubMed: 22143886) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast cell staining)
Specification
live yeast cell staining
Preparation
Cell harvesting/tissue preparation method: spin down and wash twice with water
Sample buffer: 1XPBS
Fixation
No fixation
Permeabilization
No
Gating Strategy
E tag expression positive
Username

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Verified customer

Submitted Aug 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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