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100 ug antibody/200 ul gel. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, before coupling to agarose beads. Goat anti-human E Tag affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
Our Abpromise guarantee covers the use of ab19368 in the following tested applications.
|IP||Use at an assay dependent dilution.|