Overview

  • Product nameAnti-E1 Ubiquitin Activating Enzyme antibody
    See all E1 Ubiquitin Activating Enzyme primary antibodies
  • Description
    Rabbit polyclonal to E1 Ubiquitin Activating Enzyme
  • Specificityab34711 recognises human Ubiquitin Activating Enzyme E1 protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Dog
  • Immunogen

    Recombinant fusion protein (Human) - Ubiquitin Activating Enzyme E1 (tagged).

  • Positive control
    • A431 whole cell lysate, breast, kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma).

Properties

Applications

Our Abpromise guarantee covers the use of ab34711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
IHC-P Use a concentration of 2 - 20 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IP 1/500.

Target

  • FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    ISGylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1S9 antibody
    • A1S9 protein antibody
    • A1S9T and BN75 temperature sensitivity complementing antibody
    • A1S9T antibody
    • A1ST antibody
    • AMCX1 antibody
    • CFAP124 antibody
    • CTD-2522E6.1 antibody
    • GXP 1 antibody
    • GXP1 antibody
    • MGC4781 antibody
    • POC20 antibody
    • POC20 centriolar protein homolog antibody
    • Protein A1S9 antibody
    • SMAX2 antibody
    • Uba1 antibody
    • UBA1, ubiquitin-activating enzyme E1 homolog A antibody
    • UBA1_HUMAN antibody
    • UBA1A antibody
    • UBE 1 antibody
    • UBE 1X antibody
    • UBE1 antibody
    • UBE1X antibody
    • Ubiquitin activating enzyme E1 antibody
    • Ubiquitin-activating enzyme E1 antibody
    • Ubiquitin-like modifier-activating enzyme 1 antibody
    see all

Anti-E1 Ubiquitin Activating Enzyme antibody images

  • This image shows human lung tissue stained with ab34711 at 10µg/ml.  In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.

  • ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-E1 Ubiquitin Activating Enzyme antibody (ab34711) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 35 kDa,53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-E1 Ubiquitin Activating Enzyme antibody (ab34711)

This product has been referenced in:
  • Estrada-Bernal A  et al. Functional complexity of the axonal growth cone: a proteomic analysis. PLoS One 7:e31858 (2012). WB, ICC/IF ; Rat . Read more (PubMed: 22384089) »
  • Guinez C  et al. Protein ubiquitination is modulated by O-GlcNAc glycosylation. FASEB J 22:2901-11 (2008). WB, IP ; Human . Read more (PubMed: 18434435) »

See all 2 Publications for this product

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