Recombinant
RabMAb

Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)] (ab180125)

Overview

  • Product name
    Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)]
    See all E1 Ubiquitin Activating Enzyme primary antibodies
  • Description
    Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human E1 Ubiquitin Activating Enzyme aa 200-300. The exact sequence is proprietary.
    Database link: P22314

  • Positive control
    • Fetal brain, K562, HeLa and Jurkat cell lysates; Human ovarian carcinoma tissue; Jurkat and K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab180125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa).
Flow Cyt 1/70. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/250.
ICC/IF 1/100.

Target

  • Function
    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications
    ISGylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1S9 antibody
    • A1S9 protein antibody
    • A1S9T and BN75 temperature sensitivity complementing antibody
    • A1S9T antibody
    • A1ST antibody
    • AMCX1 antibody
    • CFAP124 antibody
    • CTD-2522E6.1 antibody
    • GXP 1 antibody
    • GXP1 antibody
    • MGC4781 antibody
    • POC20 antibody
    • POC20 centriolar protein homolog antibody
    • Protein A1S9 antibody
    • SMAX2 antibody
    • Uba1 antibody
    • UBA1, ubiquitin-activating enzyme E1 homolog A antibody
    • UBA1_HUMAN antibody
    • UBA1A antibody
    • UBE 1 antibody
    • UBE 1X antibody
    • UBE1 antibody
    • UBE1X antibody
    • Ubiquitin activating enzyme E1 antibody
    • Ubiquitin-activating enzyme E1 antibody
    • Ubiquitin-like modifier-activating enzyme 1 antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.

  • Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-E1 Ubiquitin Activating Enzyme antibody [EPR14203(B)] (ab180125) at 1/5000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 118 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)

References

ab180125 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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