Overview

  • Product name
  • Description
    Rabbit polyclonal to E2 230K
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 169 of Human E2 230K (Q9C0C9)

  • Positive control
    • A431, 293T, Jurkat and Raji whole cell lysates; breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab111707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 141 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the covalent attachment of ubiquitin to other proteins.
  • Tissue specificity
    Predominantly expressed in skeletal muscle and heart.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 230K antibody
    • EC=6.3.2.19 antibody
    • FLJ12878 antibody
    • KIAA1734 antibody
    • Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme antibody
    • RP23 193A16.5 antibody
    • UBE2O antibody
    • UBE2O_HUMAN antibody
    • Ubiquitin carrier protein O antibody
    • Ubiquitin conjugating enzyme E2 O antibody
    • Ubiquitin conjugating enzyme E2 of 230 kDa antibody
    • ubiquitin conjugating enzyme E2O antibody
    • Ubiquitin protein ligase O antibody
    • Ubiquitin-conjugating enzyme E2 O antibody
    • Ubiquitin-conjugating enzyme E2 of 230 kDa antibody
    • Ubiquitin-conjugating enzyme E2-230K antibody
    • Ubiquitin-protein ligase O antibody
    see all

Images

  • Anti-E2 230K antibody (ab111707) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 141 kDa
  • Immunohistochemical analysis of paraffin-embedded breast carcinoma using ab111707 antibody at 1/500 dilution.

References

ab111707 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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