Overview

  • Product name
    Anti-E2 tag antibody [3F12]
    See all E2 tag primary antibodies
  • Description
    Mouse monoclonal [3F12] to E2 tag
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Epitope
    GVSSTSSDFRDR(linear)
  • General notes
    E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    150mM NaCl, 0.1% sodium azide, 50% glycerol
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    3F12
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    IF(ICC): 1/500- 1/1000 (1hr,RT) (dilute in 1% BSA in PBS).
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein.
    • Alternative names
      • E2 epitope tag antibody
      • SSTSSDFRDR epitope tag antibody
      • SSTSSDFRDR tag antibody

    Images

    • Staining of transfected COS-7 cells with COX-2 specific antibody (A, B, D) and with E2Tag-specific antibody (3F12)(C).

      A - pcDNA3.1 rabbit COX-2,

      B - pCG-E2Tag-COX,

      C - pCG-COX[E2Tag]

      D – neg. control, carrier DNA alone

    References

    This product has been referenced in:
    • Wu K  et al. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell 18:755-67 (2007). Read more (PubMed: 17182846) »
    • Auvinen E  et al. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol 149:1745-59 (2004). Read more (PubMed: 15593417) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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