Overview

  • Product name
    Anti-E2 tag antibody [5E11]
    See all E2 tag primary antibodies
  • Description
    Mouse monoclonal [5E11] to E2 tag
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Epitope
    SSTSSDFRDR (linear)
  • General notes
    E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    5E11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M.
ICC/IF 1/1000. Incubate for 1 hour at room temperature.

Target

  • Relevance
    The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein.
  • Alternative names
    • E2 epitope tag antibody
    • SSTSSDFRDR epitope tag antibody
    • SSTSSDFRDR tag antibody

Images

  • Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000).
  • ab977 at 1/200 dilution staining human Hela cells by ICC/IF. The HeLa cells were transfected with the fusion DNA construct pQM-NTag/A consisting of E2 tag fused in frame with gene specific cDNA. The cells were fixed with paraformaldehyde and blocked with serum prior to incubation with the antibody for 2 hours. A Cy3 conjugated sheep anti-mouse IgG antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Kaczmarek K  et al. Overexpression of peroxisomal testis-specific 1 protein induces germ cell apoptosis and leads to infertility in male mice. Mol Biol Cell 22:1766-79 (2011). Read more (PubMed: 21460186) »
  • Mannan AU  et al. Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with Reticulon 1 an endoplasmic reticulum protein. Neurogenetics 7:93-103 (2006). Read more (PubMed: 16602018) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
5 µg
Specification
HeLa
Immuno-precipitation step
Protein G
Username

Dr. Ashraf Mannan

Verified customer

Submitted Dec 22 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 30%
Username

Dr. Ashraf Mannan

Verified customer

Submitted Nov 03 2006

My apologies for the inconvenience, I will arrange for a new vial to be sent to you in your next shipment. Thank you for your patience,

I'm very sorry to hear you are having problems with ab977 lot 9057; we have not had any reported problems with this lot, it is possible the antibody has gone off when it was sent to you. I will arrange for a new vial to be sent to you as soon as possi...

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For immunoprecipitation and for affinity chromatography we recommend to use ab978 - during further elution milder conditions can be used. We have used ab978 beads for affinity purification of several different proteins in small quantities. For wes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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