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Our Abpromise guarantee covers the use of ab977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|WB||1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M.|
|ICC/IF||1/1000. Incubate for 1 hour at room temperature.|