The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similaritiesBelongs to the E2F/DP family.
Post-translational modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
All lanes : Anti-E2F1 antibody (ab55398) at 1/500 dilution
Lane 1 : HeLa cell extract, treated with Etoposide, without the immunizing peptide Lane 2 : HeLa cell extract, treated with Etoposide, with the immunizing peptide
Predicted band size : 47 kDa Observed band size : 47 kDa
References for Anti-E2F1 antibody (ab55398)
This product has been referenced in:
Nair JS et al. Aurora B kinase regulates the postmitotic endoreduplication checkpoint via phosphorylation of the retinoblastoma protein at serine 780. Mol Biol Cell20:2218-28 (2009).
Read more (PubMed: 19225156) »