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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Overview
Product name
Anti-E2F1 antibody
See all E2F1 products (20) ...
Description
Rabbit polyclonal to E2F1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1.
Positive control
293 cell line lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - E2F1 (phospho H357) antibody (ab75950)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Predicted molecular weight: 47 kDa.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similarities
Belongs to the E2F/DP family.
Post-translational
modifications
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ01094
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E2F 1 antibody
- E2F transcription factor 1 antibody
- E2F-1 antibody
see all
Database links
- Entrez Gene: 1869 Human
- Omim: 189971 Human
- SwissProt: Q01094 Human
- Unigene: 654393 Human
Anti-E2F1 antibody images:
Western blot - E2F1 (phospho H357) antibody (ab75950)
Anti-E2F1 antibody (ab75950) at 1/50 dilution + 293 cell line lysate at 35 µg
Predicted band size : 47 kDa
References for Anti-E2F1 antibody (ab75950)
ab75950 has not yet been referenced specifically in any publications.
Publishing research using ab75950? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"