Overview

  • Product nameAnti-E2F1 antibody
    See all E2F1 primary antibodies
  • Description
    Rabbit polyclonal to E2F1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 183-433 of Human E2F1 (NM_005225).

  • Positive control
    • K562 cell lysate and Human fetal tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab94888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/200.

Target

  • FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Post-translational
    modifications
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

Anti-E2F1 antibody images

  • Anti-E2F1 antibody (ab94888) at 1/500 dilution + K562 cell lysate

    Predicted band size : 47 kDa
  • ab94888, at a 1/100 dilution, staining E2F1 in formalin fixed, paraffin embedded Human fetal tonsil tissue by Immunohistochemistry.

References for Anti-E2F1 antibody (ab94888)

This product has been referenced in:
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). Read more (PubMed: 24026482) »
  • Kim SY & Rane SG The Cdk4-E2f1 pathway regulates early pancreas development by targeting Pdx1+ progenitors and Ngn3+ endocrine precursors. Development 138:1903-12 (2011). Read more (PubMed: 21490060) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"