Overview

  • Product nameAnti-E2F1 antibody [KH95] (Biotin)
    See all E2F1 primary antibodies
  • Description
    Mouse monoclonal [KH95] to E2F1 (Biotin)
  • ConjugationBiotin
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant Human E2F1 protein.

  • Epitopeaa 342-386
  • Positive control
    • Mad109 or HeLa cells. Tonsil or breast carcinomas.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/50 - 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at 2µg/mg of lysate. Native and denatured.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 47 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
    • Sequence similaritiesBelongs to the E2F/DP family.
    • Post-translational
      modifications
      Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
      Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dmel\CG6376 antibody
      • Dmel_CG6376 antibody
      • drosE2F1 antibody
      • E(Sev-CycE)3A antibody
      • E(var)3-93E antibody
      • E2-promoter binding facto antibody
      • E2F 1 antibody
      • E2F transcription factor 1 antibody
      • E2F-1 antibody
      • E2f-PA antibody
      • E2f-PB antibody
      • E2f-PC antibody
      • E2F1 antibody
      • E2f1 E2F transcription factor 1 antibody
      • E2F1_HUMAN antibody
      • Evar(3)164 antibody
      • KIAA4009 antibody
      • l(3)07172 antibody
      • l(3)j3B1 antibody
      • l(3)j3C2 antibody
      • l(3)rM729 antibody
      • mKIAA4009 antibody
      • OTTHUMP00000030661 antibody
      • PBR3 antibody
      • PRB binding protein E2F 1 antibody
      • PRB-binding protein E2F-1 antibody
      • RBAP 1 antibody
      • RBAP-1 antibody
      • RBAP1 antibody
      • RBBP-3 antibody
      • RBBP3 antibody
      • RBP 3 antibody
      • RBP3 antibody
      • Retinoblastoma-associated protein 1 antibody
      • Retinoblastoma-binding protein 3 antibody
      • Transcription factor E2F1 antibody
      see all

    References for Anti-E2F1 antibody [KH95] (Biotin) (ab79807)

    This product has been referenced in:
    • Helin K  et al. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes Dev 7:1850-61 (1993). Read more (PubMed: 8405995) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"