Anti-E2F1 (phospho S364) antibody [2] (ab122888)


  • Product name
    Anti-E2F1 (phospho S364) antibody [2]
    See all E2F1 primary antibodies
  • Description
    Mouse monoclonal [2] to E2F1 (phospho S364)
  • Specificity
    ab122888 is specific to the Human E2F1 protein phosphorylated at Ser364.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to a sequence of Human E2F1 protein including and surrounding phospho-Ser364

  • Positive control
    • MCF cells treated with etoposide at 10 µM for 16 h



Our Abpromise guarantee covers the use of ab122888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
IP Use at an assay dependent concentration.


  • Function
    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Post-translational
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

Anti-E2F1 (phospho S364) antibody [2] images

  • All lanes : Anti-E2F1 (phospho S364) antibody [2] (ab122888) at 1 µg/ml

    Lane 1 : Untreated MCF cells immunoprecipitated with a pantropic anti-E2F1 antibody
    Lane 2 : MCF cells treated with etoposide at 10 µM for 16 h, immunoprecipitated with a pantropic anti-E2F1 antibody
    Lane 3 : MCF cells treated with etoposide at 10 µM for 16 h

    Predicted band size : 47 kDa

References for Anti-E2F1 (phospho S364) antibody [2] (ab122888)

ab122888 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122888.
Please use the links above to contact us or submit feedback about this product.


Sign up