The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
FunctionTranscription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
Sequence similaritiesBelongs to the E2F/DP family.
Post-translational modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
Western blot - E2F1 (phospho S364) antibody (ab5391)
All lanes : Anti-E2F1 (phospho S364) antibody (ab5391) at 1/250 dilution
Lane 1 : CRE (mouse) cells transfected with human E2F1 Lane 2 : CRE (mouse) cells transfected with human E2F1 with doxorubicin Lane 3 : NiH3T3 (mouse) cell lysate Lane 4 : NiH3T3 (mouse) cell lysate with doxorubicin Lane 5 : MDA-231 (human breast cancer) cell lysate Lane 6 : MDA-231 (human breast cancer) cell lysate with doxorubicin