• Product nameAnti-E2F2 antibody
    See all E2F2 primary antibodies
  • Description
    Rabbit polyclonal to E2F2
  • Specificityab65222 detects endogenous levels of total E2F2 protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human E2F2.

  • Positive control
    • Extracts from HepG2 cells, K562 cells and COLO205 cells.



Our Abpromise guarantee covers the use of ab65222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


  • FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Tissue specificityHighest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Post-translational
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dE2F2 antibody
    • E2F transcription factor 2 antibody
    • E2F-2 antibody
    • E2F2 antibody
    • E2F2_HUMAN antibody
    • Transcription factor E2F2 antibody
    see all

Anti-E2F2 antibody images

  • All lanes : Anti-E2F2 antibody (ab65222) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-E2F2 antibody (ab65222)

This product has been referenced in:
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). ChIP . Read more (PubMed: 24026482) »
  • Wan Z  et al. Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors. J Clin Invest 120:3530-44 (2010). WB ; Human . Read more (PubMed: 20890043) »

See all 2 Publications for this product

Product Wall

Thank you very much for your interest in ab65222.

To our knowledge, ab65222 has not been tested in IHC or ICC. Therefore, I can offer a discount off a future purchase if you buy ab65222 now, test it inIHC or ICCand submit feedback to us in t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 40 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 24 2010