Overview

  • Product name
  • Description
    Rabbit polyclonal to E2F2
  • Specificity
    ab65222 detects endogenous levels of total E2F2 protein.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human E2F2.

  • Positive control
    • Extracts from HepG2 cells, K562 cells and COLO205 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Tissue specificity
    Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Post-translational
    modifications
    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dE2F2 antibody
    • E2F transcription factor 2 antibody
    • E2F-2 antibody
    • E2F2 antibody
    • E2F2_HUMAN antibody
    • Transcription factor E2F2 antibody
    see all

Images

  • All lanes : Anti-E2F2 antibody (ab65222) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References

This product has been referenced in:
  • Fujihara S  et al. The angiotensin II type 1 receptor antagonist telmisartan inhibits cell proliferation and tumor growth of esophageal adenocarcinoma via the AMPKa/mTOR pathway in vitro and in vivo. Oncotarget 8:8536-8549 (2017). WB . Read more (PubMed: 28052030) »
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). ChIP . Read more (PubMed: 24026482) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you very much for your interest in ab65222.

To our knowledge, ab65222 has not been tested in IHC or ICC. Therefore, I can offer a discount off a future purchase if you buy ab65222 now, test it inIHC or ICCand submit feedback to us in t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Loading amount
40 µg
Specification
Kidney
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

Submitted Dec 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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