• Product name
  • Description
    Rabbit polyclonal to E2F3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse E2F3.

    (Peptide available as ab99875.)

  • Positive control
    • This antibody gave a positive signal in Brain (Mouse) Tissue Lysate.



Our Abpromise guarantee covers the use of ab74180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).


  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C18211 antibody
    • E2F 3 antibody
    • E2F transcription factor 3 antibody
    • E2F-3 antibody
    • E2F3 antibody
    • E2F3_HUMAN antibody
    • KIAA0075 antibody
    • MGC104598 antibody
    • Transcription factor E2F 3 antibody
    • Transcription factor E2F3 antibody
    see all

Anti-E2F3 antibody images

  • Anti-E2F3 antibody (ab74180) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa

    Exposure time : 18 minutes

References for Anti-E2F3 antibody (ab74180)

This product has been referenced in:
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). ChIP . Read more (PubMed: 24026482) »

See 1 Publication for this product

Product Wall

Thank you for your reply and for keeping me updated.

I'm glad to hear that the E2F4 antibody seems to work, and that the p107 antibody is nowshowing the specific band. I ran a BLAST of the p107 immunogen sequence to see if there are other sim...

Read More

Thank you for your calls earlier this month and for your patience.

Have the replacement vials of ab53060 and ab2451 provided any new results?

I've reviewed the protocols used to test these antibodies, and I didn't notice anything pa...

Read More

Thank you for your call today and for letting us know about the trouble with these antibodies. I apologize for the inconvenience.

As we discussed, please let me know if you would like replacements for anything of these antibodies or if you mi...

Read More


Sign up