Overview

  • Product nameAnti-E2F3 antibody
    See all E2F3 primary antibodies
  • Description
    Rabbit polyclonal to E2F3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse E2F3.

    (Peptide available as ab99875.)

  • Positive control
    • This antibody gave a positive signal in Brain (Mouse) Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab74180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C18211 antibody
    • E2F 3 antibody
    • E2F transcription factor 3 antibody
    • E2F-3 antibody
    • E2F3 antibody
    • E2F3_HUMAN antibody
    • KIAA0075 antibody
    • MGC104598 antibody
    • Transcription factor E2F 3 antibody
    • Transcription factor E2F3 antibody
    see all

Anti-E2F3 antibody images

  • Anti-E2F3 antibody (ab74180) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 18 minutes

References for Anti-E2F3 antibody (ab74180)

This product has been referenced in:
  • Prystowsky M  et al. Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G2 delay and mitotic defects. Sci Rep 3:2640 (2013). ChIP . Read more (PubMed: 24026482) »

See 1 Publication for this product

Product Wall

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