The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesGSA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the E2F/DP family.
Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
Post-translational modificationsDifferentially phosphorylated in vivo.