Overview

  • Product name
  • Description
    Goat polyclonal to E2F4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human E2F4 aa 495-506 (internal sequence) (Cysteine residue). NP_001941.2
    Sequence:

    DPTGVLELPKELSE


    Database link: Q86TM6

  • Positive control
    • Human brain (cerebellum) tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab181482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).

Target

  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
  • Tissue specificity
    Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Developmental stage
    Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
  • Post-translational
    modifications
    Differentially phosphorylated in vivo.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2F 4 antibody
    • E2F transcription factor 4 antibody
    • E2F transcription factor 4 p107/p130 binding antibody
    • E2F-4 antibody
    • E2F4 antibody
    • E2F4_HUMAN antibody
    • p107/p130 binding protein antibody
    • Transcription factor E2F 4 antibody
    • Transcription factor E2F4 antibody
    see all

Images

  • Anti-E2F4 antibody (ab181482) at 3 µg/ml + Human brain (cerebellum) tissue lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 44 kDa

    Primary incubation was 1 hour.

References

ab181482 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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