Overview

  • Product name
  • Description
    Goat polyclonal to E2F4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human E2F4 aa 349-360 (Cysteine residue). NP_001941.2
    Sequence:

    PKELSEIFDPTR


    Database link: Q16254

  • Positive control
    • HeLa and A431 cell lysate, Pig brain tissue lysate, Pig heart tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab181483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).

Target

  • Function
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
  • Tissue specificity
    Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Developmental stage
    Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
  • Post-translational
    modifications
    Differentially phosphorylated in vivo.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2F 4 antibody
    • E2F transcription factor 4 antibody
    • E2F transcription factor 4 p107/p130 binding antibody
    • E2F-4 antibody
    • E2F4 antibody
    • E2F4_HUMAN antibody
    • p107/p130 binding protein antibody
    • Transcription factor E2F 4 antibody
    • Transcription factor E2F4 antibody
    see all

Images

  • All lanes : Anti-E2F4 antibody (ab181483) at 0.3 µg/ml

    Lane 1 : A431 cell lysate in RIPA buffer
    Lane 2 : HeLa cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • All lanes : Anti-E2F4 antibody (ab181483) at 0.003 µg/ml

    Lane 1 : Pig brain tissue lysate
    Lane 2 : Pig heart tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 44 kDa

References

ab181483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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