Anti-E2F4 antibody (ab53060)

Overview

  • Product nameAnti-E2F4 antibody
    See all E2F4 primary antibodies
  • Description
    Rabbit polyclonal to E2F4
  • SpecificityThis antibody detects endogenous levels of total E2F4 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from human E2F4.

  • Positive control
    • IHC-P: Human lung carcinoma tissue WB: Extracts from COS7 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
  • Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the E2F/DP family.
  • Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
  • Post-translational
    modifications
    Differentially phosphorylated in vivo.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2F 4 antibody
    • E2F transcription factor 4 antibody
    • E2F transcription factor 4 p107/p130 binding antibody
    • E2F-4 antibody
    • E2F4 antibody
    • E2F4_HUMAN antibody
    • p107/p130 binding protein antibody
    • Transcription factor E2F 4 antibody
    • Transcription factor E2F4 antibody
    see all

Anti-E2F4 antibody images

  • All lanes : Anti-E2F4 antibody (ab53060) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells
    Lane 2 : Extracts from COS7 cells treated with peptide


    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Immunohistochemical analysis of E2F4 in paraffin embedded human lung carcinoma tissue using ab53060 at a 1/50 dilution.
    Left image untreated.
    Right image treated with peptide.

References for Anti-E2F4 antibody (ab53060)

ab53060 has not yet been referenced specifically in any publications.

Product Wall

DISCOUNT CODE: xxxxxx
Expiration date: 27th January 2013

I am very pleased to hear you would like to accept our offer and testab53060 in Schmidtea Mediterranea. This code will give you: 1 freeprimary antibody or the value of ab53060 off a...

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Thank you for contacting us and your interest in our products.

Ihave had a look at the anti-E2F4 and E2F5 antibodies we have and which may be likely to cross react with the Schmidtea Mediterranea protein.

The antibody I have come ac...

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Application Western blot
Sample Mouse Cell lysate - whole cell (Retina)
Loading amount 50 µg
Specification Retina
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Apr 03 2012

Thank you for your reply and for keeping me updated.

I'm glad to hear that the E2F4 antibody seems to work, and that the p107 antibody is nowshowing the specific band. I ran a BLAST of the p107 immunogen sequence to see if there are other sim...

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Thank you for your calls earlier this month and for your patience.

Have the replacement vials of ab53060 and ab2451 provided any new results?

I've reviewed the protocols used to test these antibodies, and I didn't notice anything pa...

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Thank you for your call today and for letting us know about the trouble with these antibodies. I apologize for the inconvenience.

As we discussed, please let me know if you would like replacements for anything of these antibodies or if you mi...

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DISCOUNT CODE: ***********
Expiration date: **************

I am very pleased to hear you would like to accept our offer and test ab53060 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To red...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"