Anti-E2F4 antibody [SPM179], prediluted (ab54168)

Overview

  • Product nameAnti-E2F4 antibody [SPM179], prediluted
    See all E2F4 primary antibodies
  • Description
    Mouse monoclonal [SPM179] to E2F4, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant Human E2F4

  • Positive control
    • Raji cells, Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab54168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: ready to use.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
    • Tissue specificityFound in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similaritiesBelongs to the E2F/DP family.
    • Developmental stagePresent in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
    • Post-translational
      modifications
      Differentially phosphorylated in vivo.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2F 4 antibody
      • E2F transcription factor 4 antibody
      • E2F transcription factor 4 p107/p130 binding antibody
      • E2F-4 antibody
      • E2F4 antibody
      • E2F4_HUMAN antibody
      • p107/p130 binding protein antibody
      • Transcription factor E2F 4 antibody
      • Transcription factor E2F4 antibody
      see all

    Anti-E2F4 antibody [SPM179], prediluted images

    • immunohistochemical staining of formalin fixed, paraffin embedded human tonsil tissue section using ab54168.

    References for Anti-E2F4 antibody [SPM179], prediluted (ab54168)

    ab54168 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"