Overview

  • Product name
    Anti-E2F4 antibody [WUF11]
    See all E2F4 primary antibodies
  • Description
    Mouse monoclonal [WUF11] to E2F4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full-length protein corresponding to human E2F-4.

  • Positive control
    • K-562 nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab11951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). Uncharacterized bands of lower and higher molecular weight may also be revealed under similar conditions. For optimal results primary antibody incubations should be performed at 37°C

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
    • Tissue specificity
      Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the E2F/DP family.
    • Developmental stage
      Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
    • Post-translational
      modifications
      Differentially phosphorylated in vivo.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2F 4 antibody
      • E2F transcription factor 4 antibody
      • E2F transcription factor 4 p107/p130 binding antibody
      • E2F-4 antibody
      • E2F4 antibody
      • E2F4_HUMAN antibody
      • p107/p130 binding protein antibody
      • Transcription factor E2F 4 antibody
      • Transcription factor E2F4 antibody
      see all

    Images



    • Predicted band size : 45 kDa


      The presence of E2F-4 in K-562 nuclear extracts is confirmed by the detection of a signal corresponding to a protein of approximately 60 kD using ab11951 at a 1/1000 dilution.

    References

    ab11951 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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