Overview

  • Product name
    Anti-E2F5 antibody [220D1a]
    See all E2F5 primary antibodies
  • Description
    Mouse monoclonal [220D1a] to E2F5
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    220D1a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2F 5 antibody
    • E2F transcription factor 5 antibody
    • E2F transcription factor 5 p130 binding antibody
    • E2F-5 antibody
    • E2f5 antibody
    • E2F5_HUMAN antibody
    • p130 binding antibody
    • Transcription factor E2F5 antibody
    see all

Images

  • Anti-E2F5 antibody [220D1a] (ab59769) + immunizing peptide

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References

This product has been referenced in:
  • Wang L  et al. A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo. PLoS One 8:e85650 (2013). Read more (PubMed: 24392024) »
  • Uboldi S  et al. A systems biology approach to characterize the regulatory networks leading to trabectedin resistance in an in vitro model of myxoid liposarcoma. PLoS One 7:e35423 (2012). WB . Read more (PubMed: 22523595) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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