Anti-E2F6 antibody (ab152151)
Key features and details
- Rabbit polyclonal to E2F6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E2F6 antibody
See all E2F6 primary antibodies -
Description
Rabbit polyclonal to E2F6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 59-281 of E2F6 (UniProt ID: O75461).
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Positive control
- A549 whole cell lysate; H441 xenograft tissue; A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. -
Tissue specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen. -
Sequence similarities
Belongs to the E2F/DP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1876 Human
- Omim: 602944 Human
- SwissProt: O75461 Human
- Unigene: 603093 Human
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Alternative names
- E2F 6 antibody
- E2F binding site modulating activity protein antibody
- E2F transcription factor 6 antibody
see all
Images
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Anti-E2F6 antibody (ab152151) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin embedded H441 xenograft tissue, labeling E2F6 using ab152151 at 1/500 dilution.
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Immunofluorescence analysis of paraformaldehyde fixed A549 cells, labeling E2F6 using ab152151 at 1/500 dilution. Lower image costained with Hoerchst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab152151 has been referenced in 2 publications.
- Lu Z et al. MicroRNA-424/E2F6 feedback loop modulates cell invasion, migration and EMT in endometrial carcinoma. Oncotarget 8:114281-114291 (2017). PubMed: 29371986
- Korourian A et al. MicroRNA-31 inhibits RhoA-mediated tumor invasion and chemotherapy resistance in MKN-45 gastric adenocarcinoma cells. Exp Biol Med (Maywood) 242:1842-1847 (2017). PubMed: 28836853