The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20439742
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
ChIP - Anti-E2F6 antibody - ChIP Grade (ab53061)This image is courtesy of an anonymous Abreview
ChIP analysis using ab53061 binding E2F6 in Mouse ES cell nuclear lysate. Samples were fixed with formaldehyde for 10 mins and incubated with primary antibody for 16 hours at 4°C at an antibody concentration of 1/500. Protein binding was detected using real-time PCR at both positive and negative control regions.
Roy-Chaudhuri B et al. Regulation of microRNA-mediated gene silencing by microRNA precursors. Nat Struct Mol Biol21:825-32 (2014).
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Velasco G et al. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A107:9281-6 (2010).
Read more (PubMed: 20439742) »