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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Forkhead Box
Anti-E2F6 antibody - ChIP Grade (ab53061)
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Secondary antibodies
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab53061? Please let us know so that we can cite the reference in this datasheet
ab53061 has been referenced in 1 publications.
- Velasco G et al. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A 107:9281-6 (2010). WB, IP; Human. PubMed: 20439742
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - E2F6 antibody (ab53061)
All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilution
Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide
Predicted band size : 32 kDa
Observed band size : 32 kDa
Western blot - Anti-E2F6 antibody (ab53061)
All lanes : Anti-E2F6 antibody - ChIP Grade (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )
Lane 1 : HeLa cells
Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells
Secondary
HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 32 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Blocking Step: 4% Milk for 1 hour at 22°C
This image is courtesy of an Abreview submitted by Dr. Ligia Tavares
ChIP - Anti-E2F6 antibody - ChIP Grade (ab53061)
ChIP analysis using ab53061 binding E2F6 in Mouse ES cell nuclear lysate. Samples were fixed with formaldehyde for 10 mins and incubated with primary antibody for 16 hours at 4ºC at an antibody concentration of 1/500. Protein binding was detected using real-time PCR at both positive and negative control regions.
This image is courtesy of an anonymous Abreview
E2F6 antibody for Western blot in Mouse (53061)
Anti-E2F6 antibody for Western blot in Mouse (53061)
Anti-E2F6 antibody for ChIP in Mouse (53061)
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