Overview

  • Product name
    Anti-E2F6 antibody [TFE61]
    See all E2F6 primary antibodies
  • Description
    Mouse monoclonal [TFE61] to E2F6
  • Tested applications
    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Hela cells or K-562 nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab11952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
ICC/IF
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa from K-562 nuclear extract(predicted molecular weight: 32 kDa). For optimal results, primary antibody incubations should be performed at 37°C

    ICC/IF: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
    • Tissue specificity
      Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
    • Sequence similarities
      Belongs to the E2F/DP family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2F 6 antibody
      • E2F binding site modulating activity protein antibody
      • E2F transcription factor 6 antibody
      • E2F transcription factor 6 isoform 1 antibody
      • E2F-6 antibody
      • E2F6 antibody
      • E2F6_HUMAN antibody
      • EMA antibody
      • MGC111545 antibody
      • Transcription factor E2F6 antibody
      see all

    Images



    • Predicted band size : 32 kDa


      Detection of E2F6 by Western blot analysis. E2F6 is detected in nuclear extracts derived from K-562 cells using a 1/1000 dilution of ab11952.

    References

    This product has been referenced in:
    • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). Read more (PubMed: 26657798) »
    • Furuta M  et al. miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis 31:766-76 (2010). WB ; Human . Read more (PubMed: 19843643) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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