Overview

  • Product name
  • Description
    Goat polyclonal to E2F7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-DLSKQKKFTPERNP

    , corresponding to amino acids 53-66 of Human E2F7 (NP_976328.2).

  • Positive control
    • Human kidney, testis, heart and brain cerebellum lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

Target

  • Function
    Along with E2F8, inhibitor of E2F-dependent transcription that is important for the control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity). Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
  • Sequence similarities
    Belongs to the E2F/DP family.
  • Domain
    Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • E2F 7 antibody
    • E2F transcription factor 7 antibody
    • E2F-7 antibody
    • E2f7 antibody
    • E2F7_HUMAN antibody
    • FLJ12981 antibody
    • Transcription factor E2F7 antibody
    see all

Images

  • Anti-E2F7 antibody (ab106832) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 100 kDa
    Observed band size : 100 kDa


    Exposure time : 1 hour

References

ab106832 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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