Anti-E3 SUMO-protein ligase PIAS4 antibody [10-24.6] (ab211625)

Overview

  • Product name
    Anti-E3 SUMO-protein ligase PIAS4 antibody [10-24.6]
    See all E3 SUMO-protein ligase PIAS4 primary antibodies
  • Description
    Mouse monoclonal [10-24.6] to E3 SUMO-protein ligase PIAS4
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human E3 SUMO-protein ligase PIAS4 aa 2-473.
    Sequence:

    AAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKE LYETRYAKKN SEPAPQPHRP LDPLTMHSTY DRAGAVPRTP LAGPNIDYPV LYGKYLNGLG RLPAKTLKPE VRLVKLPFFN MLDELLKPTE LVPQNNEKLQ ESPCIFALTP RQVELIRNSR ELQPGVKAVQ VVLRICYSDT SCPQEDQYPP NIAVKVNHSY CSVPGYYPSN KPGVEPKRPC RPINLTHLMY LSSATNRITV TWGNYGKSYS VALYLVRQLT SSELLQRLKT IGVKHPELCK ALVKEKLRLD PDSEIATTGV RVSLICPLVK MRLSVPCRAE TCAHLQCFDA VFYLQMNEKK PTWMCPVCDK PAPYDQLIID GLLSKILSEC EDADEIEYLV DGSWCPIRAE KERSCSPQGA ILVLGPSDAN GLLPAPSVNG SGALGSTGGG GPVGSMENGK PGADVVDLTL DSS


    Database link: Q8N2W9

  • Positive control
    • 293T and LINCap cell lysates; 293T cells; Myc tagged E3 SUMO-protein ligase PIAS4 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab211625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500.
ICC/IF 1/250.
IP 1/50.

Target

  • Function
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificity
    Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Domain
    The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    modifications
    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localization
    Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

Images

  • All lanes : Anti-E3 SUMO-protein ligase PIAS4 antibody [10-24.6] (ab211625) at 1/2500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : LINCap cell lysate
  • Immunofluorescent analysis of 293T cells labeling E3 SUMO-protein ligase PIAS4 using ab21162 at 1/250 dilution. An Alexa Fluor 488 conjugated sheep anti mouse was used as secondary antibody.

  • Lanes 1 - 4 : anti Myc antibody
    Lane 5 :
    Lanes 6 - 10 : Anti-E3 SUMO-protein ligase PIAS4 antibody [10-24.6] (ab211625) at 1/2500 dilution

    Lane 1 : Untransfected 293T cell lysate
    Lane 2 : 293T cell lysate transfected with Myc tagged PIAS1 protein
    Lane 3 : 293T cell lysate transfected with Myc tagged PIASXbeta protein
    Lane 4 : 293T cell lysate transfected with Myc tagged PIAS3 protein
    Lane 5 : 293T cell lysate transfected with Myc tagged E3 SUMO-protein ligase PIAS4 protein
    Lane 6 : Untransfected 293T cell lysate
    Lane 7 : 293T cell lysate transfected with Myc tagged PIAS1 protein
    Lane 8 : 293T cell lysate transfected with Myc tagged PIASXbeta protein
    Lane 9 : 293T cell lysate transfected with Myc tagged PIAS3 protein
    Lane 10 : 293T cell lysate transfected with Myc tagged E3 SUMO-protein ligase PIAS4 protein

References

ab211625 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Mar 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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