Anti-E3 SUMO-protein ligase PIAS4 antibody (ab13795)

Overview

  • Product nameAnti-E3 SUMO-protein ligase PIAS4 antibody
    See all E3 SUMO-protein ligase PIAS4 primary antibodies
  • Description
    Rabbit polyclonal to E3 SUMO-protein ligase PIAS4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptides QLVQFDCSPELFKKIKE and KAVQVVLRICYS, corresponding to amino acids 44-60 and 187-198 of Human PAIAS gamma.

Properties

Applications

Our Abpromise guarantee covers the use of ab13795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificityHighly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • DomainThe LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    modifications
    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localizationNucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

Anti-E3 SUMO-protein ligase PIAS4 antibody images



  • Predicted band size : 57 kDa
  • ab13795 at 1/50 staining human fibroblasts by ICC/IF. The cells were fixed with formaldehyde and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-E3 SUMO-protein ligase PIAS4 antibody (ab13795)

This product has been referenced in:
  • Nath AK  et al. Proteomic-based detection of a protein cluster dysregulated during cardiovascular development identifies biomarkers of congenital heart defects. PLoS One 4:e4221 (2009). Read more (PubMed: 19156209) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab13795 and for taking the time to provide some useful details of the experiments as attachments. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblasts)
Specification fibroblasts
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
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Submitted Apr 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"